Molecular genotyping and epidemiology of Mycobacterium bovis strains obtained from cattle in Iran

Restriction fragment length polymorphism (RFLP) genotyping was employed to analyze the population genetics of Mycobacterium bovis in Iran. One hundred and twenty-three isolates collected from slaughtered tuberculosis-suspect cattle and one clinically asymptomatic buffalo were subjected to RFLP analysis with probes of the polymorphic GC-rich sequence (PGRS) and the direct repeat sequence (DR) using DNA digested with PvuII and AluI. All these methods detected a large homogeneous population in which only a few isolates had variant genotypes. Only AluI-based RFLPs of both the PGRS and DR sequences were able to clearly differentiate between BCG and field strains of M. bovis. As in previous reports, these findings seem to reflect a recent dispersal of one or a few strains in Iran following the substantial expansion of Holstein-Friesian cattle over the last few decades.     

A gamma‐ray spectrometry approach to field separation of illuviation‐type WRB reference soil groups in northern Thailand

According to the World Reference Base for Soil Resources (IUSS Working Group WRB, 2006), the differentiation of Acrisols and Alisols is based on the cation‐exchange capacity of clay, which cannot be directly determined in the field, but needs expensive and time‐consuming soil‐chemical analyses. This is an unsatisfactory situation for pedologists, who urgently require a rapid field method to distinguish illuviation‐type reference soil groups (Alisols, Acrisols, Luvisols, Lixisols). In this study, we tested the ability of gamma‐ray spectrometry to separate major WRB reference soil groups in the field. The underlying hypothesis is that Alisols and Acrisols are distinguished by their clay mineral composition, which should be reflected by geochemistry and consequently gamma‐ray radiation (i.e., K‐containing illite vs. K‐free kaolinite). Highly significant differences in their gamma‐ray spectrum for K, Th, and U were found for limestone and its soils. Especially the K and Th signatures allowed a clear separation of Acrisols and Alisols. In general, the surface radiation was sufficient to separate these soils. Best results were revealed considering parent rock and the whole soil profile. This means by using a portable radiometer and a pH meter, all illuviation‐type reference soil groups could be distinguished in this case. If applicable at other sites, this approach could enormously reduce expenditures for soil‐chemical analysis needed to assist soil classification.     

Estimation of additive and non-additive genetic effects for fertility and reproduction traits in North American Holstein cattle using genomic information

Non-additive genetic effects are usually ignored in animal breeding programs due to data structure (e.g., incomplete pedigree), computational limitations and over-parameterization of the models. However, non-additive genetic effects may play an important role in the expression of complex traits in livestock species, such as fertility and reproduction traits. In this study, components of genetic variance for additive and non-additive genetic effects were estimated for a variety of fertility and reproduction traits in Holstein cattle using pedigree and genomic relationship matrices. Four linear models were used: (a) an additive genetic model; (b) a model including both additive and epistatic (additive by additive) genetic effects; (c) a model including both additive and dominance effects; and (d) a full model including additive, epistatic and dominance genetic effects. Nine fertility and reproduction traits were analysed, and models were run separately for heifers (N = 5,825) and cows (N = 6,090). For some traits, a larger proportion of phenotypic variance was explained by non-additive genetic effects compared with additive effects, indicating that epistasis, dominance or a combination thereof is of great importance. Epistatic genetic effects contributed more to the total phenotypic variance than dominance genetic effects. Although these models varied considerably in the partitioning of the components of genetic variance, the models including a non-additive genetic effect did not show a clear advantage over the additive model based on the Akaike information criterion. The partitioning of variance components resulted in a re-ranking of cows based solely on the cows’ additive genetic effects between models, indicating that adjusting for non-additive genetic effects could affect selection decisions made in dairy cattle breeding programs. These results suggest that non-additive genetic effects play an important role in some fertility and reproduction traits in Holstein cattle.     

Variation in autosomal and sex-linked genetic effects for growth traits in Markhoz goat using multivariate animal models

Tropical Animal Health and Production - The present study was conducted to estimate autosomal and sex-linked genetic parameters by restricted maximum likelihood method using four different...     

Evaluation of pan coefficient equations for estimating reference crop evapotranspiration in the arid region

Reference evapotranspiration (ET₀) can be estimated on basis of pan evaporation data (E_pan), whose measurements have the advantage of low cost, simplicity of the measuring equipment, simple data interpretation and application as well as suitability for locations with limited availability of meteorological data. E_pan values were converted to ET₀ using the pan evaporation coefficient (K_pan). In this study, seven common K_pan equations were evaluated for prediction of ET₀ in the growing season (April to October) in arid region of Iran. The Cuenca approach was best suited compared to the standard FAO Penman–Monteith method (FAO-56 PM).     

Calibration of Hargreaves–Samani equation for estimating reference evapotranspiration in semiarid and arid regions

The Penman–Monteith (FAO-56 PM) equation is suggested as the standard method for estimating evapotranspiration (ET₀) by the International Irrigation and Drainage Committee and Food and Agriculture Organization (FAO). On the other hand, the Hargreaves–Samani (HS) equation is an alternative method compared with the FAO-56 PM equation. In the present study, the original coefficient C of the HS equation is calibrated based on the FAO-56 PM equation for estimating the reference ET₀ from 15 meteorological stations in central Iran (about 170,000 km²) under semiarid and arid conditions. After calibration, the new values for C are ranged from 0.0018 to 0.0037. The mean bias error (MBE), the root mean square error (RMSE), and the ratio of average estimations of ET₀ (R) values for all stations are ranged from 0.12 to 5.38, ?5.35 to 1.15 mm d^?1 and 0.64 to 1.28 for the HS equation and from 0.12 to 2.48, ?2.2 to 0.60 mm d^?1, and 1.00 to 1.05 for the calibrated Hargreaves–Samani equation (CHS), respectively. Results indicate that the average RMSE and MBE values are decreased by 40% and 66%, respectively. Relationships for calibrating the C coefficient on the basis of annual average of daily temperature range (ΔT) and wind speed (V) are proposed, calibrated, and validated. Hence, the CHS equation can be used for ET₀ estimates with acceptable accuracy instead of the FAO-56 PM method.     

Optimizing water,nitrogen and crop density in canola cultivation using response surface methodology and central composite design

Abstract

Optimisation of water and nitrogen use is an effective management tool to conserve resources and reduce environmental pollutions. Response surface methodology (RSM) is defined as a collection of mathematical and statistical methods that are used to develop, to improve or to optimize a product or process. In order to determine optimum levels of water, nitrogen and planting density of canola (Brassica napus L.), a 2-year experiment (2010–2011) was carried out by central composite design as RSM at the research station of Ferdowsi University of Mashhad. The treatments were designed based on low and high levels of irrigation (1500 and 4000 m³ ha^?1), nitrogen (0 and 400 kg N ha^?1) and density (50 and 150 plant m^?2) as independent variables. Furthermore, seed yield, nitrogen losses, nitrogen use efficiency (NUE) and water use efficiency (WUE) were measured as response variables in a full quadratic polynomial model. Optimum levels of irrigation, nitrogen and planting density were suggested to achieve the target range of dependent variables based on three scenarios: economic, environmental and eco-environmental. The results showed that increasing irrigation and fertilizer led to an increase in seed yield and nitrogen losses, whereas increasing canola density resulted in an increase in seed yield but a decrease in nitrogen losses. The optimum levels of water, fertilizer and density based on environmental scenario were 1802 m³ ha^?1, 11 kg N ha^?1 and 122 plant m^?2, respectively. To achieve optimum conditions under the economic scenario, it is necessary to use 3411 m³ water ha^?1, 178 kg N ha^?1 and 119 plant m^?2. Amounts of 2347 m³ water ha^?1, 92 kg N ha^?1 and 114 plant m^?2 were found to be the optimum conditions for the eco-environmental scenario. In general, it seems that resource use based on the eco-environmental scenario may be the most favorable cropping strategy for canola production.     

Pulp Fiber Residue and Supplemental Irrigation on Yield and Nutrient Uptake of Potato

ABSTRACT

Organic matter levels combined with drought periods during the growing season have adverse impacts on rain-fed potato production in coarse textured soils of eastern Canada. Using pulp fiber residues (PFR) may build up soil organic matter, improve crops yield and nutrient uptake, and reduce the need for supplemental irrigation in this region. A box experiment with 3 rates of PFR [0, 45, and 90 Mg ha^{? 1} dry mater carbon (C) basis] with or without supplementary drip irrigation was established in a sandy loam soil in New Brunswick, Canada. Red clover, barley, and potato were cultivated as three crops of the rotation in 1999, 2000, and 2001, respectively. The PFR only applied before red clover in fall 1998. Selected soil chemical and physical properties were measured before planting and after harvest. Crops yield and nitrogen (N), phosphorus (P), and potassium (K) accumulation were determined at harvest. Soil organic-C content, bulk density and water holding capacity were improved by PFR application. Red clover and barley biomass production were significantly increased by PFR application. This effect was promoted by supplemental irrigation. Potato tuber yield was not affected by PFR and it was higher in rain-fed compared to supplementary irrigated system. Although, there was some initial evidence of N immobilization but generally PFR application increased N, P, and K bioavailability. This research has demonstrated that PFR can successfully be used as soil amendment in coarse textured soils without any adverse effect.     

Effects of Coenzyme Q10 on the ratio of TH1/TH2 in Experimental Autoimmune Encephalomyelitis Model of Multiple Sclerosis in C57BL/6

Background: Multiple sclerosis (MS) is known as a progressive central nervous system inflammatory disease. Certain factors, such as interleukins, inflammatory cells, and oxidative stress are supposed to involve in MS etiology. Because of the important role of oxidative stress, antioxidant therapy for MS has received more attention. Although coenzyme Q10 (CoQ10) acts as an antioxidant, there is a lack of enough research on its effects on MS. Therefore, the present research was designed. Methods: C57BL/6 female adult mice (n = 30) were used in this study. The animals were randomly divided into trial and control groups. To induce MS, routine procedure for experimental autoimmune encephalomyelitis (EAE) was used, and scoring was performed based on clinical signs. By detecting score one, CoQ10 administration was started (10 mg/kg/three weeks). By using ELISA and real-time PCR, the brain levels of TNF-, IL-10, IL-4, and IL-12 were studied. Statistical tests were used to analyze the data and the P value less than 0.05 was considered to be significant. Results: Clinical symptoms in EAE animals were significantly decreased (P<0.05) as compared to control ones. In addition, the level of the TNF- was significantly decreased following CoQ10 administration versus IL-10. The ratio of TH1/TH2 interleukins in treated animals was significantly less than that in non-treated animals (P<0.01). Conclusion: Our findings showed that CoQ10 is capable of suppressing the inflammatory pathway of MS. Key Words: Experimental autoimmune encephalomyelitis (EAE), Multiple Sclerosis (MS), Coenzyme Q10 (CoQ10)     

Evaluation of Water and Nitrogen Use Efficiency,Digestibility and Some Quantitative and Qualitative Characteristics of Forage Beet Cultivars Under Different Irrigation Methods and Nitrogen Levels

The aim of this study was to evaluate the water and nitrogen use efficiency and some quantitative and qualitative characteristics of forage beet cultivars under the influence of different irrigation methods and nitrogen levels in two cropping years, 2017–18 and 2018–19, at Agricultural Research Station in Karaj, Iran. Experimental factors included the first factor with four irrigation methods (normal leakage, alternate furrow irrigation, fixed furrow irrigation, type (drip-strip)), the second factor was the amount of nitrogen fertilizer with three levels (150, 200 and 250?kg N ha^?1) and the third factor included three forage beet cultivars (Sbsi052, Jamon and Kyros). Among irrigation treatments, alternate furrow irrigation and fixed furrow irrigation had the highest sugar content with 9.28% and 9.17%, respectively. The highest yield of digestible organic matter was obtained in leakage irrigation treatment, nitrogen fertilizer of 250?kg ha^?1 and in Kyros at the rate of 19.45?t ha^?1. The highest yield of root digestible dry matter, potassium, sodium and free nitrogen was observed in leakage irrigation treatment and consumption of 200?kg ha^?1 nitrogen was observed in foreign cultivars. The highest crude protein was observed in alternate furrow irrigation conditions with a consumption of 200?kg ha^?1 nitrogen in cultivar Sbsi052 at 13.08%. Leakage irrigation and type tape had the highest consumption efficiency and efficiency of nitrogen uptake with application of 150 and 200?kg ha^?1 N, and the highest water use efficiency was also observed in leakage irrigation and type tape with application of 250?kg ha^?1 N in domestic and foreign cultivars. The type irrigation method showed better quantitative and qualitative yield than the furrow irrigation methods.